Studies on the body growth and free amino acids in plasm and liver of rat fed on sucrose diet adding Methionine, Threonine and Tryptophane

Methionine, Threonine, Tryptophane을 添加한 砂糖飼料에 의한 白쥐의 體重增加 및 Plasm, 肝臟中의 遊離아미노酸에 대한 硏究

By the micro-bioassay method, quantitative studies were made on the increment of body weight and changes in free-lysine and threonine contents in the liver and plasm of rats, when they were fed on sucrose diet added with 0.3% DL-methionine, 0.36% DL-threonine and 0.1% L-tryptophane. The results of the experiment were as follows: (1) The weight gain and growth gain of rats decreased more when they were fed on 0.36% DL-threonine diet (Sth-group) than 0.3% DL-methionine diet (Sm-group). But those gain were increased when they were fed on 0.1% L-tryptophane diet (Str-group). (2) The contents of free-lysine and threonine in plasm and liver of rats were decreased more when they were fed on 0. 36% DL-threonine diet than 0.3% DL-methionine diet. And, those free amino acids were increased greatly when they were fed on 0.1% L-tryptophane diet. (3) The increment of body weight was the biggest when the free amino acid pattern showed that the contents of free-lysine decreased and threonine increased in the plasm and liver of rats being fed on 0.l% L-tryptophane diet.