Studies on the Posterior Pituitary Hormones I. The Preparation and Assay of the Posterior Pituitary Hormones

뇌하수체 후엽홀몬에 관한 연구 (I) 후엽홀몬의 조제와 그 역가검정

Oxytocin and vasopressin were extracted from bovine posterior pituitaries and assayed, in many ways. On the assay of oxytocic active substance, it was found the depression method of blood pressure in a chicken was the easiest one among possible methods. The potency of oxytocin which was extracted with glacial acetic acid was 14.2 I.U./mg. On the assay of vasopressin for pressor activity. A full grown healthy male rat was used. Applying a simple artificial respiratory apparatus, the assay could be carried out successfully. The potency of vasopressin was 13.2 I.U/mg.