Proceedings of the Plant Resources Society of Korea Conference (한국자원식물학회:학술대회논문집)
- 2022.09a
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- Pages.31-31
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- 2022
Free Radical Scavenging and Antioxidant Activities of Water Extracts from Amannia multiflora, Amannia coccinea, Salix gracilistyla Inhabiting Along the Nakdong River (Republic of Korea)
- Jayasingha Arachchige Chathuranga Chanaka Jayasingha (Department of Marine Life Science, Jeju National University) ;
- Mi-Hwa Lee (Nakdonggang National Institute of Biological Resources) ;
- Chang-Hee Kang (Nakdonggang National Institute of Biological Resources) ;
- Yung Hyun Choi (Department of Biochemistry, College of Korean Medicine Dong-Eui University) ;
- Gi-Young Kim (Department of Marine Life Science, Jeju National University)
- Published : 2022.09.27
Abstract
Plant-derived antioxidants are used as a healthy diet and are known to inhibit various human diseases. In this study, we investigated free radical scavenging and antioxidant activity of extracts from three plants (Ammannia multiflora, Ammannia coccinea and Salix gracilistyla) with the most DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity from 196 plant extracts inhabiting along Nakdong River in Republic of Korea. The three extracts also have strong total antioxidant activity. Moreover, the extracts inhibited hydrogen peroxide (H2O2)-induced reactive oxygen species production and depolarized mitochondrial membrane potential in RAW264.7 macrophages. In zebrafish larvae, 2',7'-dichlorodihydrofluorescein diacetate (DCFDA) fluorescent intensity, induced by H2O2, was markedly reduced by the extracts of A. multiflora, A. coccinea and S. gracilistyla. Meanwhile, the extracts were upregulated Nrf2 and HO-1 expression, and an HO-1 inhibitor reversed the extract-induced oxidative responses both in vivo and in vitro. The data suggest that the extracts of A. multiflora, A. coccinea, and S. gracilistyla exert potential free radical scavenging and antioxidant capacities both in vivo and in vitro by activating the Nrf2/HO-1 signaling pathway.