Selection of Low Lignin-high Biomass Whole Crop Silage Rice Elite Line for the Improvements of Forage Digestibility and Fermentation

  • Eok-Keun Ahn (Central Area Crop Breeding Division, National Institute of Crop Science, RDA) ;
  • Jeom-Ho Lee (Central Area Crop Breeding Division, National Institute of Crop Science, RDA) ;
  • Hyang-Mi Park (Central Area Crop Breeding Division, National Institute of Crop Science, RDA) ;
  • Yong-Jae Won (Cheorwon Branch, National Institute of Crop Science, RDA) ;
  • Kuk-Hyun Jeong (Chuncheon Branch, National Institute of Crop Science, RDA) ;
  • Ung-Jo Hyun (Central Area Crop Breeding Division, National Institute of Crop Science, RDA) ;
  • Yoon-Sung Lee (Central Area Crop Breeding Division, National Institute of Crop Science, RDA)
  • Published : 2022.10.13

Abstract

Lignin modification has been a breeding target for the improvements of forage digestibility and fermentation in whole crop silage(WCS) rice. In rice, gold hull and internode 2 (gh2) was identified as a lignin-deficient mutant. gh2 exhibits a reddish-brown pigmentation in the hull and the internode is located on the short arm of chromosome 2 and codes for cinnamyl-alcohol dehydrogenase (CAD). To develop WCS rice variety improved digestibility and fermentation, we measured acid detergent fiber (ADF), lignin and total digestible nutrient (TDN) calculated from ADF (TDN=88.9-(0.79% × ADF) and performed marker-assisted selection using CAD(Os2g0187800) gene first intron region specific marker with 55 Jungmo1038/J.collection lines. Those lines had lignin content range from 0.82 to 6.61%, ADF from 15.8 to 45.8%, TDN from 52.7 to 78.8 compared to 'Jungmo1038'(1.53,20.7,72.6), 'J.collection'(0.98,12.8,78.8%) and gh2 were introgressed into 44 lines. Considering on these genotype and low-lignin phenotype, we finally selected 2 elite lines(Suweon668, Suweon669). Suweon668 and Suweon669 line are high biomass-low lignin lines that the ADF content is relatively low, even though the dry matter weight is high. Also they have lodging and shattering resistance and glabrous leaf and hull important to improve cattle palatability. Our results will provide that rice can be improved for forage digestibility and fermentation with low lignin concentration.

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Acknowledgement

Supported by a project grant from "Cooperative Research Program for Agriculture Science & Technology Development (Project title: Development of multi-purpose rice cultivars based on the Saltol QTL, Project No. PJO16752032022)" Rural Development Administration, Republic of Korea