Proceedings of the Korean Society of Crop Science Conference (한국작물학회:학술대회논문집)

The Korean Society of Crop Science (한국작물학회)
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Studies on QTLs for Bakanae Disease Resistance with Populations Derived from Crosses between Korean japonica Rice Varieties

  • Dong-Kyung Yoon (Gene Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences) ;
  • Chaewon Lee (Gene Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences) ;
  • Kyeong-Seong Cheon (Gene Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences) ;
  • Yunji Shin (Gene Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences) ;
  • Hyoja Oh (Gene Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences) ;
  • Jeongho Baek (Gene Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences) ;
  • Song-Lim Kim (Gene Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences) ;
  • Young-Soon Cha (Gene Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences) ;
  • Kyung-Hwan Kim (Gene Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences) ;
  • Hyeonso Ji (Gene Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences)
  • Published : 2022.10.13
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Abstract

Rice bakanae disease is a serious global threat in major rice-cultivating regions worldwide causing high yield loss. It is caused by the fungal pathogen Fusarium fujikuroi. Varying degree of resistance or susceptibility to bakanae disease had been reported among Korean japonica rice varieties. We developed a modified in vitro bakanae disease bioassay method and tested 31 Korean japonica rice varieties. Nampyeong and Samgwang varieties showed highest resistance while 14 varieties including Junam and Hopum were highly susceptible with 100% mortality rate. We carried out mapping QTLs for bakanae disease resistance with four F2:F3 populations derived from the crosses between Korean japonica rice varieties. The Kompetitive Allele-Specific PCR (KASP) markers developed in our laboratory based on the SNPs detected in Korean japonica rice varieties were used in genotyping F2 plants in the populations. We found four major QTLs on chromosome 1, 4, 6, and 9 with LOD scores of 21.4, 6.9, 6.0, and 60.3, respectively. In addition, we are doing map-based cloning of the QTLs on chromosome 1 and 9 which were found with Junam/Nampyeong F2:F3 population and Junam/Samgwang F2:F3 population, respectively. These QTLs will be very useful in developing bakanae disease resistant high quality rice varieties.

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Acknowledgement

본 연구는 키다리병 신규 저항성 유전자 분리 및 기능 검정 사업(사업번호:PJ014985012022)의 지원에 의해 이루어진 결과로 이에 감사드립니다.