Proceedings of the Plant Resources Society of Korea Conference (한국자원식물학회:학술대회논문집)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Genotoxicity Evaluation of the Glycyrrhiza New Variety extract

감초 신품종 추출물의 유전독성 평가

  • Young-Jae Song (Department of Oriental Medicine Resources, Jeonbuk National University) ;
  • Dong-Gu Kim (Department of Oriental Medicine Resources, Jeonbuk National University) ;
  • Jeonghoon Lee (Herbal Crop Research Division, NIHHS, RDA) ;
  • Wonnam Kim (Cnh Center for Cancer Research) ;
  • Hyo-Jin An (Department of Pharmacology, College of Korean Medicine, Sangji University) ;
  • Jong-Hyun Lee (Department of Pharmacy, College of Pharmacy, Dongduk Women's University) ;
  • Jaeki Chang (Crop Production & Physiology Division, NICS, RDA) ;
  • Sa-Haeng Kang (Department of Oriental Medicine Resources, Jeonbuk National University) ;
  • Yong-Deok Jeon (Department of Korean Pharmacy, College of Pharmacy, Woosuk University) ;
  • Jong-Sik Jin (Department of Oriental Medicine Resources, Jeonbuk National University)
  • 송영재 (전북대학교 환경생명자원대학 한약자원학과) ;
  • 김동구 (전북대학교 환경생명자원대학 한약자원학과) ;
  • 이정훈 (농촌진흥청 국립원예특작과학원 약용작물과) ;
  • 김원남 (Cnh 암의학연구소) ;
  • 안효진 (상지대학교 한의과대학 약리학교실) ;
  • 이종현 (동덕여자대학교 약학대학 약학과) ;
  • 장재기 (농촌진흥청 국립식량과학원 작물재배생리과) ;
  • 강사행 (전북대학교 환경생명자원대학 한약자원학과) ;
  • 전용덕 (우석대학교 약학대학 한약학과) ;
  • 진종식 (전북대학교 환경생명자원대학 한약자원학과)
  • Published : 2021.04.29
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Abstract

The genus Glycyrrhiza (Licorice) has been used as an oriental herbal medicine for a long time in Asian countries. Wongam (WG), which is Glycyrrhiza new variety, have been developed to improve limitation of licorice including low productivity, environmental restriction and insufficient components by Korea Rural Development Administration. To using WG as a herbal medicine, it is important to reveal the adverse effects in health. In this study, we evaluated the genotoxicity test of WG extract through in vitro bacterial reverse mutation (AMES) assay, in vitro chromosomal aberration assay and in vivo mouse bone marrow micronucleus assay. When compared with the control, WG extract with or without the S9 mix showed no genotoxicity in the AMES assay up to 5000 ㎍/plate and in the chromosomal aberration assay up to 1100 ㎍/ml. In micronucleus assay, no significant increase in the number of micronucleated polychromatic erythrocytes or in the mean ratio of polychromatic to total erythrocytes up to 5000 mg/kg/day for 2 days. The present study demonstrated that WG extract is safe and reliable herbal medicine since no detectable genotoxic effects at least under the conditions of this study.

Keywords

Acknowledgement

This work was carried out with the support of "Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01424602)" Rural Development Administration, Republic of Korea.