Acknowledgement
This research was supported by funding from the Korea Forestry Promotion Institute (KOFPI, no. 2020205C10-2022-BA01).
Proceedings of the Plant Resources Society of Korea Conference (한국자원식물학회:학술대회논문집)
- 2020.12a
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- Pages.47-47
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- 2020
Complete Genome Sequence and Analysis of Carnation Italian Ringspot Virus from Erigeron annuus (L.) Pers. in Korea
- Chung Youl Park (Division of Wild Plant Seeds Research, Baekdudaegan National Arboretum) ;
- Da Hyun Lee (Division of Wild Plant Seeds Research, Baekdudaegan National Arboretum) ;
- Young Ho Jung (Division of Wild Plant Seeds Research, Baekdudaegan National Arboretum) ;
- JunHyeok Kim (Division of Wild Plant Seeds Research, Baekdudaegan National Arboretum) ;
- Mi Hyun Lee (Division of Wild Plant Seeds Research, Baekdudaegan National Arboretum) ;
- Un Seop Shin (Division of Wild Plant Seeds Research, Baekdudaegan National Arboretum) ;
- Hee Ho Lee (Division of Wild Plant Seeds Research, Baekdudaegan National Arboretum) ;
- Cho Hee Park (Division of Wild Plant Seeds Research, Baekdudaegan National Arboretum) ;
- Chae Sun Na (Division of Wild Plant Seeds Research, Baekdudaegan National Arboretum)
- Published : 2020.12.11
Abstract
In this study, we aimed to study carnation italian ringspot virus (CIRV) in Erigeron annuus (L.) Pers. in Bonghwa County, Korea. The collected samples showed mosaic and malformation symptoms. To identify the virus species, we performed high-throughput sequencing, reverse transcription polymerase chain reaction, and cloning. The virus was confirmed to be an unreported species, and therefore we performed genome sequencing of the samples. The complete genome was 4,746 nucleotides in length. The CIRV contained five open reading frames (ORFs), and it showed the typical features of members of the genus Tombusvirus. Phylogenetic analyses revealed that ClRV isolates had the highest nucleotide identities with the CZ isolate (95.89%) from Korea. In recent years, these viruses have sporadically been reported in floral scent and medicinal plants. This research found the first natural host infected with CIRV, and provides baseline information to determine the correlation between weeds and crops.