Acknowledgement
Grant : Development and application of cryopreservation technique for strawberry and Lilum germplasm and quality management for seed base collection
Supported by : National Institute of Agricultural Sciences
This study describes an efficient and widely applicable droplet-vitrification following cryopreservation for shoot tips of (Fragaria x ananassa DUCH. cvs. 'Derunoka' and 'Jumbo pure berry'. The shoot tips of strawberry were precultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.7M). Precultured explants were treated with loading solution (LS, C4) containing glycerol 17.5% and sucrose 17.5% for 40 min and exposed to dehydration solution (B1) containing 50% of glycerol and 50% of sucrose for 60 min at
Grant : Development and application of cryopreservation technique for strawberry and Lilum germplasm and quality management for seed base collection
Supported by : National Institute of Agricultural Sciences