Proceedings of the Korean Society of Crop Science Conference (한국작물학회:학술대회논문집)
- 2017.06a
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- Pages.191-191
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- 2017
Study on hydroxy fatty acid contents changes and physiological responses under abiotic stresses in transgenic Camelina
- Kim, Hyun-Sung (Department of Bioenergy Science and Technology, College of Agriculture and Life Sciences, Chonnam National University) ;
- Lee, Hyun-Sook (Department of Bioenergy Science and Technology, College of Agriculture and Life Sciences, Chonnam National University) ;
- Lim, Hyun-Gyu (Department of Bioenergy Science and Technology, College of Agriculture and Life Sciences, Chonnam National University) ;
- Park, Won (Bioenergy Crop Research Institute, National Institute of Crop Science) ;
- Kim, Hyun-Uk (Department of Bioindustry and Bioresource Engineering, Plant Engineering Research Institute, Sejong University) ;
- Lee, Kyeong-Ryeol (Department of Agricultural Biotechnology National Institute of Agricultural Science, Rural Development Administration) ;
- Ahn, Sung-Ju (Department of Bioenergy Science and Technology, College of Agriculture and Life Sciences, Chonnam National University)
- Published : 2017.06.04
Abstract
Hydroxy fatty acid (HFA) is an important industrial resource that known to be extracted from seeds of Castor or Lesquerella. However, mass production of HFA from those crops are difficult because of their behavior or life cycle. In this study, we applied HFA synthesis related gene FAH12, RcPDAT1, RcLPCAT, RcDGAT2, and RcPDCT on bioenergy crop Camelina sativa. Furthermore, we determined NaCl or cold stress tolerance changes of transgenic Camelina. RcFAH12, RcPDAT1, RcLPCAT, RcDGAT2, and RcPDCT genes were cloned into multigene expression vector which is engineered with seed specific promoter of FAE1 or Napin. Combination of HFA genes multi-expression vector constructs were divided into Set3 (RcFAH12, RcPDAT1-2, RcLPCAT), Set4 (RcFAH12, RcDGAT2, RCPDAT1-2, RcLPCAT), and Set5 (RcFAH12, RcDGAT2, RCPDAT1-2, RcLPCAT, RcPDCT). Transgenic HFA synthesis Camelina plants were generated using agrobacterium-mediated vacuum infiltration system. Results of fatty acid composition of T1 transgenic Camelina seeds analyzed by GC-MS showed 9.5, 9.0, and 13.6 % of HFA contents in Set3#6, Set4#8, and Set5#10, respectively. Therefore, seeds of T2 generation were harvest from Set5#10 which is shown highest HFA contents, and, 17.7, 8.1 and 10.5 % of HFA contents were determined in Set5#10-5, Set5#10-8, and Set#10-10, respectively. However, 7.7% of C18:2 and 22.3 % of C18:3 among unsaturated fatty acids were decreased in Set5#10-5 than WT. Meanwhile, we confirmed abiotic stress responses in T2 transgenic Camelina Set5#10-5 and Set5#10-10 under 0, 100, 150, and 200 mM NaCl or 25, 15, and