Proceedings of the Korean Institute of Information and Commucation Sciences Conference (한국정보통신학회:학술대회논문집)

The Korea Institute of Information and Commucation Engineering (한국정보통신학회)
권호 표지

Demyelination of Myelinated Neuronal cells by Infection of Herpes Simplex Virus-1

Herpes Simplex Virus-1감염에 의한 수초화 뉴우런의 탈수초

  • Kim, Hyun Joo (Department of Anesthesiology and Pain Medicine, Yonsei University College of Medicine) ;
  • Kim, Ji-Young (Department of Anesthesiology and Pain Medicine, Yonsei University College of Medicine) ;
  • Sa, Young-Hee (Department of Anesthesiology and Pain Medicine, Yonsei University College of Medicine) ;
  • Hong, Seong-Karp (Division of Bio and Health Sciences, Mokwon University)
  • Published : 2016.10.27
Download PDF
⟨ Previous Next ⟩

Abstract

Neuronal cells and Schwann cells from dorsal root ganglion (DRG) in embryos of rat were isolated and cultured in vitro respectively. The purified neuronal cells added with anti-mitotic agents and purified Schwann cells were co-cultured and then accomplished myelination processing. This myelinated co-culture system was infected by herpes simplex virus-1 and then accomplished demyelination processing in this myelinated co-culture. We identified myelination and demyelination processing using antibody of neuropeptide Y meaning presence of myelinated neuron.

쥐의 배아의 척수신경절로 부터 슈반세포와 뉴런 세포가 각각 분리되어 배양되었다. in vitro 시스템에서 배양되었다. 정제된 뉴런 세포는 항 유사분열제로 처리하였고 정제한 슈반세포들과 함께 공동배양 하였다. 이 때 수초화 과정이 진행되었다. 이렇게 수초화된 공동 배양에 Herpes simplex virus-1을 감염시켜 탈수초화를 진행 시켰다. 우리는 수초화의 존재를 의미하는 neuropeptide Y의 항체를 이용하여 수초화와 탈수초화를 확인하였다.

Keywords