Proceedings of the Korean Institute of Information and Commucation Sciences Conference (한국정보통신학회:학술대회논문집)
- 2015.05a
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- Pages.954-957
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- 2015
Comparison of Recombinant Baculovirus Vector Systems and Control Vector System
재조합 베큘로바이러스벡터와 대조 벡터의 비교
- Kim, Ji-Young (Yonsei University College of Medicine) ;
- Hong, Seong-Karp (Mokwon University)
- Published : 2015.05.26
Abstract
A recombinant baculovirus vector systems were composed of genes of polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP), and protein transduction domain (PTD). These recombinant baculovirus vector system were transfected into various cell lines and tissues and confirmed gene transfer and expression of these vector systems with only control vector system. From the result, gene transfer and gene expression of recombinant baculovirus vector systems were superior in terms of efficacy and safety than in the control vector system.
polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP), protein transduction domain (PTD) 유전자로 구성된 재조합 베큘로바이러스를 제작하였다. 본 재조합 베큘로바이러스 시스템은 여러가지 세포주와 여러 가지 조직에 감염하여 시험하였고 재조합된 유전자의 전달과 유전자 발현을 대조 벡터시스템과 비교하였다. 본 연구의 결과로 제작된 재조합 베큘로바이러스 시스템은 유전자의 전달과 발현에 있어서 대조 벡터시스템 보다 효능과 안전성면에서 우수하였다.
Keywords
- baculovirus;
- enhanced green fluorescent protein;
- protein transduction domain;
- vesicular stomatitis virus G