Proceedings of the Korean Vacuum Society Conference (한국진공학회:학술대회논문집)
- 2013.08a
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- Pages.268.2-268.2
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- 2013
Detection of PspA by Interdigitated Nanogap Devices
- Park, Jimin (Department of Chemistry, Sungkyunkwan University (SKKU)) ;
- Park, Dae Keun (Department of Chemistry, Sungkyunkwan University (SKKU)) ;
- Lee, Cho Yeon (Department of Chemistry, Sungkyunkwan University (SKKU)) ;
- Kang, Aeyeon (Department of Chemistry, Sungkyunkwan University (SKKU)) ;
- Oh, Jihye (Department of Biological Science, Sungkyunkwan University (SKKU)) ;
- Kim, Gyuhee (Department of Biological Science, Sungkyunkwan University (SKKU)) ;
- Lee, Sangho (Department of Biological Science, Sungkyunkwan University (SKKU)) ;
- Yun, Wan Soo (Department of Chemistry, Sungkyunkwan University (SKKU))
- Published : 2013.08.21
Abstract
Nanogap interdigitated electrodes (NIDEs) can serve as an alternative platform for the biomolecular detection [1]. In this work, the NIDEs were adopted in a simple and sensitive detection of Pneumococcal surface protein A (PspA). The NIDEs were fabricated by the combination of photo and chemical lithography. Photolithographically-defined initial gap of about 200 nm was narrowed down to a few tens of nanometers by surface-initiated growth of the initial electrodes (chemical lithography) [2]. Bare silicon oxide surface between the electrodes was chemically modified to immobilize capturing antibodies and, after exposure to the samples, the device was immersed in a solution containing the probe-antibody-conjugated Au nanoparticles (Au NPs). The conductance change accompanied with the Au NP immobilization was interpreted as the existence of PspA. Detection limit of the measurements and further improvement of the detection efficiency were discussed with the results from I-V analysis, scanning electron microscopy, and atomic force microscopy.
Keywords
- Nanogap interdigitated electrodes;
- Pneumococcal surface protein A (PspA);
- Chemical lithography;
- Antibody-conjugated Au nanoparticles