한국약용작물학회:학술대회논문집

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Rapid Identification of the ${\alpha}$-Glucosidase Inhibitory Compounds from Geranium thunbergii Sieb. et Zucc. by HPLC-micro-fractionation and HPLC-UV-$MS^n$

  • Choi, Se-Jin (Department of Food Science and Nutrition, Hallym University) ;
  • Kim, Jin-Kyu (Institute of Natural Medicine, Hallym University) ;
  • Lee, Yeon-Sil (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University) ;
  • Lim, Soon-Sung (Department of Food Science and Nutrition, Hallym University)
  • Published : 2010.10.12
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Abstract

In this study ethanol extracts of aerial part of Geranium thunbergii Sieb. et Zucc. was investigated for their ability to inhibit a-glucosidase, and thus was fractionated using two organic solvents, including dichloromethane, ethyl acetate. The ethyl acetate-soluble fraction, which manifested potent enzyme inhibitory properties, was then followed by tracking down the active compound by combining HPLC micro-fractionation to an enzyme assay in 96-well plate. The ${\alpha}$-glucosidase inhibitory activity profile showed that two peaks exhibited potent inhibitory activity, and then the structural analyses of the two peaks were carried out by HPLC-UV-MS. The main ${\alpha}$-glucosidase inhibitory compounds in the ethyl acetate-soluble fractions of ethanol extracts of Geranium thunbergii Sieb. et Zucc. were tentatively identified as geraniin and kaempferol-7-rhamnoside.

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