Proceedings of the Korean Vacuum Society Conference (한국진공학회:학술대회논문집)
- 2010.02a
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- Pages.44-45
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- 2010
Micro-Spot Atmospheric Pressure Plasma Production for the Biomedical Applications
- Hirata, T. (Department of Biomedical Engineering, Tokyo City University) ;
- Tsutsui, C. (Nano Carbon Bio Device Research Center, Tokyo City University) ;
- Yokoi, Y. (Department of Biomedical Engineering, Tokyo City University) ;
- Sakatani, Y. (Department of Biomedical Engineering, Tokyo City University) ;
- Mori, A. (Department of Biomedical Engineering, Tokyo City University) ;
- Horii, A. (Department of Electrical and Electronic Engineering, Tokyo City University) ;
- Yamamoto, T. (Department of Electrical and Electronic Engineering, Tokyo City University) ;
- Taguchi, A. (Department of Biomedical Engineering, Tokyo City University)
- Published : 2010.02.17
Abstract
We are currently conducting studies on culturing and biocompatibility assessment of various cells such as neural stem cells and induced pluripotent stem cells(IPS cells) on carbon nanotube (CNT), on nerve regeneration electrodes, and on silicon wafers with a focus on developing nerve integrated CNT based bio devices for interfacing with living organisms, in order to develop brain-machine interfaces (BMI). In addition, we are carried out the chemical modification of carbon nanotube (mainly SWCNTs)-based bio-nanosensors by the plasma ion irradiation (plasma activation) method, and provide a characteristic evaluation of a bio-nanosensor using bovine serum albumin (BSA)/anti-BSA binding and oligonucleotide hybridization. On the other hand, the researches in the case of "novel plasma" have been widely conducted in the fields of chemistry, solid physics, and nanomaterial science. From the above-mentioned background, we are conducting basic experiments on direct irradiation of body tissues and cells using a micro-spot atmospheric pressure plasma source. The device is a coaxial structure having a tungsten wire installed inside a glass capillary, and a grounded ring electrode wrapped on the outside. The conditions of plasma generation are as follows: applied voltage: 5-9 kV, frequency: 1-3 kHz, helium (He) gas flow: 1-1.5 L/min, and plasma irradiation time: 1-300 sec. The experiment was conducted by preparing a culture medium containing mouse fibroblasts (NIH3T3) on a culture dish. A culture dish irradiated with plasma was introduced into a
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