한국전산유체공학회:학술대회논문집
- 2008.03b
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- Pages.581-584
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- 2008
Generation of sheath-free particle beam: application to micro-flow cytometry
외피유체 없이 입자 빔의 발생: 유세포 분류기 응용
- Kim, Young-Won (Institute of Advanced Machinery and Design, Seoul National University) ;
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Yoo, Jung-Yul
(School of Mechanical and Aerospace Engineering, Seoul National University)
- Published : 2008.03.26
Abstract
A generation of a particle beam is the key technique in a flow cytometry that measures the fluorescence and light scattering of individual cell and other particulate or molecular analytes in biomedical research. Recent methods performing this function require a laborious and time-consuming assembly. In the present work, we propose a novel device for the generation of an axisymmetrical focusing beam of microparticles (3-D focusing) in a single capillary without sheath flows. This work uses the concept that the particles migrate toward the centerline of the channel when they lag behind the parabolic velocity profile. Particle focusing of spherical particles was successfully made with a beam diameter of about 10
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