대한전기학회:학술대회논문집 (Proceedings of the KIEE Conference)
- 대한전기학회 2008년도 제39회 하계학술대회
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- Pages.1466-1467
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- 2008
유체와 온도 조절을 이용한 생화학 물질 반응용 마이크로칩의 개발
BEAD BASED CHEMICAL REACTION SYSTEM USING TEMPERATURE AND FLUID CONTROL FOR CANCER DETECTION
- 김민수 (서울대 전기,컴퓨터공학부) ;
- 이보람 (서울대 화학생물공학부) ;
-
윤효진
(서울대 화학생물공학부) ;
- 김병기 (서울대 화학생물공학부) ;
-
이윤식
(서울대 화학생물공학부) ;
-
김용권
(서울대 전기,컴퓨터공학부)
- Kim, Min-Su (School of Electrical Engineering and Computer Science, Seoul National University) ;
- Lee, Bo-Rahm (School of Chemical & Biological Engineering, Seoul National University) ;
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Yoon, Hyo-Jin
(School of Chemical & Biological Engineering, Seoul National University) ;
- Kim, Byung-Gee (School of Chemical & Biological Engineering, Seoul National University) ;
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Lee, Yoon-Sik
(School of Chemical & Biological Engineering, Seoul National University) ;
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Kim, Yong-Kweon
(School of Electrical Engineering and Computer Science, Seoul National University)
- 발행 : 2008.07.16
초록
We describe here a novel micro total analysis system for the purification and identification of the affinity-captured proteins. Also we demonstrated the mass analysis of the Carcinoembrionic antigen (CEA) and Alpha femtoprotein which were chosen as the target cancer marker. For MALDI-TOF analyses, the proteins should to be separated from a protein mixture and be concentrated when needed. This procedure usually takes a long time even before protease-digested samples are to be obtained from them. Here, we describe integrated and efficient micro chip for protein purification and digestion for MALDI-TOF analyses. At first, disease protein is purified by passing the micro chamber from a protein mixture or human whole serum and released from the micro affinity beads by thermal heating. Purified protein is then transfer to the hole for trypsin digestion. The final sample is analyzed by MALDI-TOF. All the processes could be finished successfully within one hour, which renders MALDI-TOF analyses of a target protein quite simple.
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