대한전기학회:학술대회논문집 (Proceedings of the KIEE Conference)

대한전기학회 (The Korean Institute of Electrical Engineers)
권호 표지

펩타이드 질량 분석을 위한 전기 이온화 분사기의 제작 및 성능 평가

Mass spectrometry analysis system with integrated micro electrospray ionization emitter for peptide detection

  • 김민수 (서울대학교 전기 컴퓨터 공학부) ;
  • 주황수 (서울대학교 화학생물공학부) ;
  • 김병기 (서울대학교 화학생물공학부) ;
  • 김용권 (서울대학교 전기 컴퓨터 공학부)
  • Kim, Min-Su (School of Electrical Engineering and Computer Science, Seoul National University) ;
  • Joo, Hwang-Soo (School of Chemical Engineering and Institute of Molecular Biology and Genetics, Seoul National University) ;
  • Kim, Byung-Gee (School of Chemical Engineering and Institute of Molecular Biology and Genetics, Seoul National University) ;
  • Kim, Yong-Kweon (School of Electrical Engineering and Computer Science, Seoul National University)
  • 발행 : 2007.07.18
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초록

This paper describes a novel microfluidic device with a microfabricated electrospray source for a sheathless electrospray ionization interface to a mass spectrometer. This electrospray ionization-mass spectrometry (ESI-MS) device consists of a triangular-shaped metal emitter, allowing the generation of an efficient electrospray for peptide detection, and microfluidic channels monolithically in a glass microchip. The performance of the proposed interface was evaluated by opimizing its experimental condition and spraying standard peptides. The spraying has high signal strength and stability, with a relative standard deviation of 2.9% and singly-charged and doubly-charged peaks of the peptides were successfully detected. The metal emitter source showed a good performance to be comparable to commercially available emitters in signal strength and stability.

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