Proceedings of the KIEE Conference (대한전기학회:학술대회논문집)

The Korean Institute of Electrical Engineers (대한전기학회)
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Fabrication and Electrochemical Detection Property of Single Strand DNA Hybridization Sensor

DNA Hybridization 센서의 제작과 전기화학적 검출 특성

  • Lee, Dong-Yun (Department of Electrical Engineering & NTRC, Dong-A University) ;
  • Yang, Chang-Heon (Department of Electrical Engineering & NTRC, Dong-A University) ;
  • Choi, Won-Suk (Department of Nano Engineering & NTRC, Dong-A University) ;
  • Park, Sang-Hyun (Department of Electrical Engineering & NTRC, Dong-A University) ;
  • Kwon, Young-Soo (Department of Electrical Engineering & NTRC, Dong-A University)
  • 이동윤 (동아대학교 전기공학과 & NTRC) ;
  • 양창헌 (동아대학교 전기공학과 & NTRC) ;
  • 최원석 (동아대학교 나노공학과 & NTRC) ;
  • 박상현 (동아대학교 전기공학과 & NTRC) ;
  • 권영수 (동아대학교 전기공학과 & NTRC)
  • Published : 2007.07.18
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Abstract

A synthesized 21-mer single-stranded DNA(ss DNA) was covalently immobilized onto a self-assembled aminoethanethiol monolayer modified gold electrode onto QCM. The covalently immobilized ssDNA was hybridized with complementary ssDNA. The interaction between surface immobilized ssDNA and complementary 21-mer DNA in solution was also examined. Each step was followed by monitoring changes in the QCM frequency with time. Also, PBS with pH 7.0 was selected as a supporting electrolyte in order to get maximum sensitivity and good bioactivity.

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