한국전기전자재료학회:학술대회논문집 (Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference)
- 한국전기전자재료학회 2006년도 영호남 합동 학술대회 및 춘계학술대회 논문집 센서 박막 기술교육
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- Pages.65-70
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- 2006
Hoechst groove binder를 이용한 유전자의 전기화학적 검출
Electrochemical Gene Detection Using Hoechat Groove Binder
- Choi, Yong-Sung (Department of Electrical Engineering, DongShin University) ;
- Lee, Woo-Ki (Department of Electrical Engineering, DongShin University) ;
- Lee, Kyung-Sup (Department of Electrical Engineering, DongShin University)
- 발행 : 2006.05.19
초록
In this study, a DNA chip with a microelectrode array was fabricated using microfabrication technology. Several probe DNAs consisting of mercaptohexyl moiety at their 5 end were immobilized on the gold electrodes by DNA arrayer. Then target DNAs were hybridized and reacted with Hoechst 33258, which is a DNA minor groove binder and electrochemically active dye. Linear sweep voltammetry or cyclic voltammetry showed a difference between target DNA and control DNA in the anodic peak current values. It was derived from Hoechst 33258 concentrated at the electrode surface through association with formed hybrid. It suggested that this DNA chip could recognize the sequence specific genes.