Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference (한국전기전자재료학회:학술대회논문집)
- 2006.06a
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- Pages.410-411
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- 2006
SNP Detection Using Indicator-free DNA Chip
비수식화 DNA를 이용한 유전자 검출
- Choi, Yong-Sung (Department of Electrical Engineering, DongShin University) ;
- Moon, Jong-Dae (Department of Electrical Engineering, DongShin University) ;
- Lee, Kyung-Sup (Department of Electrical Engineering, DongShin University)
- Published : 2006.06.22
Abstract
High throughput analysis using a DNA chip microarray is powerful tool in the post genome era. Less labor-intensive and lower cost-performance is required. Thus, this paper aims to develop the multi-channel type label-free DNA chip and detect SNP (Single nucleotide polymorphisms). At first, we fabricated a high integrated type DNA chip array by lithography technology. Various probe DNAs were immobilized on the microelectrode array. We succeeded to discriminate of DNA hybridization between target DNA and mismatched DNA on microarray after immobilization of a various probe DNA and hybridization of label-free target DNA on. the electrodes simultaneously. This method is based on redox of an electrochemical ligand.
Keywords
- DNA chip microarray;
- SNP (Single nucleotide polymorphisms);
- Lithography technology;
- Hybridization;
- Immobilization