한국고분자학회:학술대회논문집 (Proceedings of the Polymer Society of Korea Conference)

한국고분자학회 (The Polymer Society of Korea)
권호 표지

Bio-inert Surface of Pluronic-immobilized Flask for Preservation of Hematopoietic Stem Cells

  • Higuchi, Akon (Department of Materials & Life Science, Seikei University) ;
  • Aoki, Nobuo (Department of Materials & Life Science, Seikei University) ;
  • Gomei, Yumiko (Department of Materials & Life Science, Seikei University) ;
  • Matsuoka, Yuki (Department of Materials & Life Science, Seikei University)
  • 발행 : 2006.10.13
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초록

Human umbilical cord blood was stored at $4^{\circ}C$ in the Pluronic-immobilized flask as well as commercially available bio-inert flasks, and flow cytometric analysis of surface markers was performed on hematopoietic stem cells after cultivation. The number of cells expressing $CD34^{+}$ in umbilical cord blood on the Pluronic-immobilized flask was extremely higher than those obtained using other flasks. It is concluded that the flexible and hydrophilic segments of Pluronic conjugated on the flask surface are the reason for the effective preservation of hematopoietic stem cells in the Pluronic-immobilized flask.

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