Proceedings of the Korean Society for Food Science of Animal Resources Conference (한국축산식품학회:학술대회논문집)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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A Simple Method for the Detection and Identification of Korean Brown Cattle(Hanwoo) using DHPLC

  • Kang, Won (Dept of chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) ;
  • Park, Sane-Bum (Dept of chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) ;
  • Nam, Youn-Hyoung (Dept of chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) ;
  • An, Young-Chang (Dept of chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) ;
  • Lee, Sang-Hyun (Dept of chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) ;
  • Park, Jin-Keun (Dept of chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) ;
  • Lee, Jae-Sik (Dept of chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) ;
  • Jang, On-Cheoul (Dept of chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) ;
  • Park, Su-Min (Genetbio, Chungnam Animal Science Center) ;
  • Park, Yong-Hyun (Genetbio, Chungnam Animal Science Center) ;
  • Ko, Kyung-Hee (Chungnam Animal Science Center, Konyang Univ.) ;
  • Moon, Sung-Sil (Chungnam Animal Science Center, Konyang Univ.)
  • Published : 2006.05.26
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Abstract

Denaturing high-performance liquid chromatography(DHPLC) is used in a wide variety of genetic applications and is an efficient method for detection of mutations involving one or a few nucleotides. We developed a high-throughput DHPLC method for identifying polymorphisms in the MC1R gene that are characteristic of Hanwoo cattle. We compared 10 tissue samples from Hanwoo cattle, 10 samples from Holstein cattle and 10 samples from Hanwoox Holstein crossbred cattle to determine whether DHPLC analysis can be used to distinguish between these genotypes. Samples obtained from Hanwoo cattle had a unique profile of peaks that could be used as a molecular fingerprint for this breed. We also analyzed two hundred samples in a trial in which we were blinded to the genotype of the samples and correctly identified the breed-of-origin of 594 out of 600 sequence variations(99%).

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