Proceedings of the Korean Aquaculture Society Conference (한국양식학회:학술대회논문집)
- 2006.05a
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- Pages.476-478
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- 2006
Determination of subcellular localization of Betanodavirus B2
Abstract
To analyze subcellular localization of betanodavirus protein B2, a plasmid expressing Betanodavirus protein B2 fused to enhanced green fluorescent protein (EGFP-Nl) was constructed. The transient expression of full-length B2 fused to EGFP in GF cells confirmed the equal distribution of protein B2 between cytoplasm and nucleus. However, transfection of N-terminal half of the B2 revealed that this truncated form predominantly localized to the cytoplasm. By using several deletion mutants and point mutants, we determined the regions and/or motif responsible for the subcellular localization of betanodavirus.
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