한국생물공학회:학술대회논문집
- 한국생물공학회 2005년도 생물공학의 동향(XVII)
- /
- Pages.889-893
- /
- 2005
Comparison of various cell lysis methods for Pichia pastoris
- Han, Kyung-Ah (Department of Material and Biochemical Engineering, BioProcess Technology lab., Research Center for BioPhotonics, Chonnam National University) ;
- Kim, Sun-Yong (Faculty of Applied Chemical Engineering, BioProcess Technology lab., Research Center for BioPhotonics, Chonnam National University) ;
- Rhee, Jong-Il (Faculty of Applied Chemical Engineering, BioProcess Technology lab., Research Center for BioPhotonics, Chonnam National University)
- 발행 : 2005.10.27
초록
10 g/L yeast extract, 20 g/L peptone, 20 g/L dextrose가 첨가된 YPD 배지를 이용하여 200 rpm, 30
Cell lysis method is very important to study intracellular metabolites in microorganisms. In this study, various cell lysis methods were compared to find a good method analysing intracellular metabolites in P. pastoris. P. pastoris was cultivated in YPD medium at 30
키워드