한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Development a glucose-FIA system with a fiber optic oxygen sensor

  • Sohn, Ok-Jae (Department of Materal and Biochemical Engineering, BioProcess Technology lab., Research Center for BioPhotonics, Chonnam National University) ;
  • Lam, Tuan-Hung (BioProcess Technology lab., Research Center for BioPhotonics, Chonnam National University) ;
  • Rhee, Jong-Il (Faculty of Applied Chemical Engineering, BioProcess Technology lab., Research Center for BioPhotonics, Chonnam National University)
  • Published : 2005.10.27
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Abstract

Flow injection analysis (FIA) system was developed to monitor glucose concentrations in biotechnological processes. A fiber optic oxygen sensor was used to determine consumption of oxygen concentration by reaction of immobilized glucose oxidase (GOD). The GOD was immobilized on VA-Epoxy carrier and integrated into FIA system. A calibration curve for glucose was obtained in the range of 0.5 $g/L{\sim}3.0$ g/L.

본 연구에서는 광섬유 산소센서를 기반으로 글루코오스 모니터링용 FIA 시스템을 개발하였다. 운반용액의 낮은 유속에서도 검출가능 하였으며, 산소전극을 이용한 글루코오스-FIA 시스템보다 넓은 범위의 글루코오스 농도를 검출할 수 있었다. 향후, 광섬유 센서를 이용하여 생물반응기에서 기질로 사용되는 글루코오스뿐만 아니라 용존산소, pH, $CO_2$와 같이 다양한 인자들의 모니터링 시스템을 개발하고자 한다.

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