한국생물공학회:학술대회논문집
- 한국생물공학회 2005년도 생물공학의 동향(XVII)
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- Pages.511-516
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- 2005
Response Surface Methodological Approach for Optimization of Enzymatic Synthesis of Sorbitan Methacrylate
- Jeong, Gwi-Taek (School of Biological Sciences and Technology) ;
- Lee, Kyoung-Min (School of Biological Sciences and Technology) ;
- Kim, Hae-Sung (Dept. of Chemical Engineering, Myongji University) ;
- Lee, Woo-Tai (Faculty of Applied Chemical Engineering) ;
- Sunwoo, Chang-Shin (School of Biological Sciences and Technology, Faculty of Applied Chemical Engineering) ;
- Park, Don-Hee (School of Biological Sciences and Technology, Faculty of Applied Chemical Engineering, Institute of Bioindustrial Technology, Chonnam National University)
- 발행 : 2005.10.27
초록
Sorbitan methacrylate was synthesized from sorbitan dehydrated from D-sorbitol using an immobilized lipase. To optimize the enzymatic synthesis of sorbitan methacrylate, response surface methodology was applied to determine the effects of five-level-four-factors and their reciprocal interactions on sorbitan methacrylate biosynthesis. A total of 30 individual experiments were performed, which were designed to study reaction temperature, reaction time, enzyme amount and substrate molar ratio. A statistical model predicted that the highest conversion yield of sorbitan methacrylate was 100%, at the following optimized reaction conditions: a reaction temperature of 43.06
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