한국식물생명공학회:학술대회논문집 (Proceedings of the Korean Society of Plant Biotechnology Conference)
- 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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- Pages.356-360
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- 2005
RNAi-mediated reduction of xanthine dehydrogenase results in increased biomass of Arabidopsis seedlings
- Nakagawa, Ayami (Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University) ;
- Sakamoto, Atsushi (Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University) ;
- Takahashi, Misa (Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency) ;
- Morikawa, Hiromichi (Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency)
- 발행 : 2005.11.02
초록
Xanthine dehydrogenase (XDH), a classic enzyme involved in purine catabolism, can catalyze the formation of redox-signaling reactive oxygen and nitrogen species such as superoxide and nitric oxide. We generated transgenic plants of Arabidopsis in which XDH was knocked out by introduction of hairpin RNA-expression vector. Expression analysis by reverse transcription-PCR and in-gel staining of XDH activity revealed that transgenic lines efficiently suppressedXDH expression at the transcriptional level, demonstrating that RNA interference was successfully induced. XDH-suppressed transgenic lines exhibitedincreased biomass production during the growth of seedlings.
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