한국생물공학회:학술대회논문집
- 2005.04a
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- Pages.408-411
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- 2005
Expression of various fluorescent protein and their production in shake flasks
- Park, So-Jung (Faculty of Applied Chemical Engineering, Bioprocess Technology lab., Chonnam National University) ;
- Han, Kyung-Ah (Department of Material and Biochemical Engineering, Bioprocess Technology lab., Chonnam National University) ;
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Rhee, Jong-Il
(Faculty of Applied Chemical Engineering, Bioprocess Technology lab., Chonnam National University)
- Published : 2005.04.15
Abstract
The green fluorescent protein (GFP) from the jellyfish aequorea and its fluorescent homologs from Anthozoa corals have become invaluable tools for imaging of cells and tissues. In this study various fluorescent protein such as green fluorescent protein (GFP), yellow fluorescent protein (YFP) and red fluorescent protein (RFP) have been expressed in Escherichia coli. Growth of recombinant cells and production of fluorescent proteins were investigated in shake flasks. Some characteristics of fluorescent proteins was also studied.
대장균을 이용하여 형광단백질을 형질전환하고 배양하면서 그 특성을 관찰하고 형광세기 및 형광 단백질의 크기를 조사하였다.
Keywords