한국생물공학회:학술대회논문집
- 한국생물공학회 2005년도 생물공학의 동향(XVI)
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- Pages.210-213
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- 2005
Effect of Anti-apoptotic Agents in Recombinant Chinese Hamster Ovary Cell Expressing Iduronate 2-sulphatase
- Jeon, Ok-Seon (Division of Biotechnology, Kangwon National University) ;
- Chun, Ju-Me (STR biotech) ;
- Kang, Seon-Ah (Division of Biotechnology, Kangwon National University) ;
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Lee, Sang-Jong
(STR biotech) ;
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Chun, Gie-Taek
(Division of Life Science, Kangwon National University) ;
- Chang, Yong-Keun (Department of Chemical Engineering and BioProcess Engineering Research Center, KAIST) ;
- Jeong, Yeon-Ho (Division of Biotechnology, Kangwon National University)
- 발행 : 2005.04.15
초록
본 연구에서는 세포 사멸을 억제시켜 재조합 단백질의 생산을 향상시키기 위한 전략을 개발하기 위해, 우선 STR-G와 EGCG를 농도별로 첨가하여 세포 성장, 사멸 그리고 IDS 생산성에 미치는 영향을 조사하였다. Flow cytometry 분석 결과, STR-G에 의한 apoptotic cell percentage의 감소, STR-G와 EGCG에 의한 bel-2 expression level의 증가와 IDS 생산성의 증가를 확인하였다.
The suppression of apoptosis during the cell culture might increase recombinant protein production. In the present study, the effects of anti-apoptotic agents on the apoptosis of recombinant Chinese Hamster Ovary cells and the production of Iduronate 2-sulphatase(IDS) were investigated Cell density slightly increased when
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