한국미생물학회:학술대회논문집 (Proceedings of the Microbiological Society of Korea Conference)
- 한국미생물학회 2005년도 International Meeting of the Microbiological Society of Korea
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- Pages.132-134
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- 2005
Development of the Phage Displayed Peptide as an Inhibitor of MCP-1 (Monocyte Chemoattractant Protein-1)-mediated Angiogenesis
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Jeong, Sun-Joo
(Department of Molecular Biology, Institute of nanosensor and Biotechnology, Dankook University)
- 발행 : 2005.05.12
초록
The CC chemokine, monocyte chemoattractant protein-1 (MCP-1), plays a crucial role in the initiation of atherosclerosis and has direct effects that promote angiogenesis. To develop a specific inhibitor for MCP-1-induced angiogenesis, we performed in vitro selection employing phage display random peptide libraries. Most of the selected peptides were found to be homologous to the second extracellular loops of CCR2 and CCR3. We synthesized the peptide encoding the homologous sequences of the receptors and tested its effect on the MCP-1 induced angiogenesis. Surface Plasmon Resonance measurements demonstrated specific binding of the peptide to MCP-1 but not to the other homologous protein, MCP-3. Flow cytometry revealed that the peptide inhibited the MCP-1 binding to THP-1 monocytes. Moreover, CAM and rat aortic ring assays showed that the peptide inhibited MCP-1 induced angiogenesis. Our observations indicate that the MCP-1-binding peptide exerts its anti-angiogenic effect by interfering with the interaction between MCP-1 and its receptor.
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