Proceedings of the IEEK Conference (대한전자공학회:학술대회논문집)

The Institute of Electronics and Information Engineers (대한전자공학회)
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ANALYSIS OF FLUIDIC BEAD CUBE EMBEDDED PORTABLE CMOS SENSING SYSTEM FOR IMMUNO REACTION MONITORING

유체소자가 집적화된 면역검사용 휴대용 CMOS 바이오칩의 분석

  • Jeong, Yong-Won (School of Electrical Engineering and Computer Science, Seoul National University) ;
  • Park, Se-Wan (School of Electrical Engineering and Computer Science, Seoul National University) ;
  • Kim, Jin-Seok (School of Electrical Engineering and Computer Science, Seoul National University) ;
  • Kim, Hyeon-Cheol (School of Electrical Engineering and Computer Science, Seoul National University) ;
  • Chun, Kuk-Jin (School of Electrical Engineering and Computer Science, Seoul National University)
  • 정용원 (서울대학교 공과대학 전기컴퓨터공학부) ;
  • 박세완 (서울대학교 공과대학 전기컴퓨터공학부) ;
  • 김진석 (서울대학교 공과대학 전기컴퓨터공학부) ;
  • 김현철 (서울대학교 공과대학 전기컴퓨터공학부) ;
  • 전국진 (서울대학교 공과대학 전기컴퓨터공학부)
  • Published : 2005.11.26
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Abstract

This paper describes the novel immunoassay sensing system for a portable clinical diagnosis system. It consists of a bead cage reactor and a CMOS integrated biosensor. It showed the simple and easy antibody coating method on beads by flow-through avidin biotin complex technology in a microfluidic device. It showed just 90 nL sample consumption and good result for the application of alpha feto protein. The bead cage reactor has the role of the antibody coating, antigen binding and enzyme linking for the electrochemical sensing method. The CMOS biosensor consists of ISFET (ion selective field effect transistor) biosensor and temperature sensor for detecting pH that is the byproduct of enzyme reaction. The sensitivity is 8 $kHz/^{\circ}C$ in a temperature sensor and 33 mV/pH in a pH sensor. After filling the 15 um polystyrene beads in bead cage, antibody flowed and reacted to beads. Subsequently, the biotinylated antigen flowed and bound to the antibody and GOD (glucose oxidase)-avidin conjugate flowed and reacted to the biotin of the biotinylated antigen. After this reaction process, glucose solution flowed and reacted to the GOD on beads. The hydrogen was generated by glucose-GOD reaction. And it was detected by the pH sensor.

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