Universal-, Genus-specific, Species-specific Probes and Primers Design for Microbial Identification

  • Park, Jun-Hyung (Busan Genome Cente, College of Medicine, Pusan National University) ;
  • Park, Hee-Kyung (Institute for Genomics Medicine, GeneIn. Co., Ltd.) ;
  • Song, Eunsil (Institute for Genomics Medicine, GeneIn. Co., Ltd.) ;
  • Jang, Hyun-Jung (Institute for Genomics Medicine, GeneIn. Co., Ltd.) ;
  • Kang, Byeong-Chul (Division of Applied Bioengineering, Dongseo University) ;
  • Lee, Seung-Won (Busan Genome Cente, College of Medicine, Pusan National University) ;
  • Kim, Hyun-Jin (Busan Genome Cente, College of Medicine, Pusan National University) ;
  • Kim, Cheol-Min (Busan Genome Cente, College of Medicine, Pusan National University)
  • Published : 2005.09.22

Abstract

MIPROBE is a web-based tool for design of universal, genus-specific, and species-specific primers and probes. The main functions of MIPROBE are collection of target gene sequences, construction of consensus sequences, collection of candidate primers and probes, and evaluation of candidates by BLAST. Biologists with little computer skills can easily use MIPROBE to design large-scale universal, genus-, and species-specific primers and probes. This software is available at http://www.miprobe.com. Also detailed descriptions of how to use the program are found at this site.

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