한국식물생명공학회:학술대회논문집 (Proceedings of the Korean Society of Plant Biotechnology Conference)
- 한국식물생명공학회 2004년도 생명공학 실용화를 위한 비젼
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- Pages.62-65
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- 2004
Development of transgenic disease-resistant root stock for the growth of watermelon
- Cho, Song-Mi (Applied Plant Science Division, College of Agriculture and Life Science, Chonnam National University, PhytoCareTech Co., LTD.) ;
- Chung, Soo-Jin (Applied Plant Science Division, College of Agriculture and Life Science, Chonnam National University) ;
- Moon, Sun-Jin (PhytoCareTech Co., LTD.) ;
- Kim, Kwang-Sang (PhytoCareTech Co., LTD.) ;
- Kim, Young-Cheol (Applied Plant Science Division, College of Agriculture and Life Science, Chonnam National University) ;
- Cho, Baik-Ho (Applied Plant Science Division, College of Agriculture and Life Science, Chonnam National University)
- 발행 : 2004.10.22
초록
To protect the watermelon against soil-borne pathogens, we are currently producing disease-resistant transgenic root stock for the growth of watermelon, A defensin gene (J1-1) from Capsicum annum, a ACC deaminase gene from Pseudomonas syringae, a galactinol synthase (CsGolS) gene from Cucumis sativus, and a WRKY (CvWRKY2) gene from Citullus vulgaris were used as transgenes for disease resistance. The gene were transformed into a inbred line (6-2-2) of watermelon, Kong-dae watermelon and a inbred line (GO702S) of gourd, respectively, by Agrobacterium-mediated transformation. Putative transgenic plants were selected in medium containing 100mg/L kanamycin, and then integration of the genes into the genomic DNA were demonstrated by PCR analysis. Successful integration of the gene in regenerated plants was also confirmed by PCR (Figf 1), genomic Southern blot (Fig 2), RT-PCR (Fig 3), and Northern blot analysis(Fig 4). Several T1 lines having different transgene were produced, and disease resistance of the T1 lines are under estimation.
키워드