Proceedings of the Korean Environmental Health Society Conference (한국환경보건학회:학술대회논문집)

Korean Society of Environmental Health (한국환경보건학회)
권호 표지

A new sensitive determination method of propylene oxide-hemoglobin adducts by EI-GC-MS (SIM)

  • Shin, Ho-Sang (Department of Environmental Education, Abused Drug Research Center, Kongju National University) ;
  • Lee, Jin-Heon (Department of Environmental Education) ;
  • Ahn, Hye-Sil (Department of Environmental Education)
  • Published : 2004.06.04
Download PDF
⟨ Previous Next ⟩

Abstract

A gas chromatography/mass spectrometric assay method was developed for the determination of propylene oxide adduct, N-(3-hydroxypropyl)valine. Adduct was released from hemoglobin by alkaline hydrolysis and extract at pH 8 with ethyl ether. The dried extract was completely derivatized with N-Methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA)/TMS-I (100:3). The detection limits of the assay were 0.08 ng/g for N-(3-hydroxypropyl)valine based upon assayed hemoglobin of 0.1 g. The method was applied to the determination of propylene oxide adduct formed in young female Sprague-Dawley rats after treatment for 1, 2 and 3 weeks with 0.008 % propylene oxide via the drinking water. An adduct was detected by proposed procedure. The structure of the adduct could be assigned to N-(3-hydroxypropyl)valine.

Keywords