한국산학기술학회:학술대회논문집 (Proceedings of the KAIS Fall Conference)
- 한국산학기술학회 2004년도 추계학술대회
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- Pages.299-301
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- 2004
쌀겨로부터 lipase 효소의 정제 및 생화학적인 분석
Purification and Biochemical Analysis of Rice Bran Lipase Enzyme
- 김영희 (생명대학교 공과대학 생명정보공학과)
- Kim Younghee (Dept. of Biotechnology and informatics, College of Engineering, Sangmyung University,)
- 발행 : 2004.11.01
초록
A simple procedure for the extraction of the lipolytic enzyme from rice bran has been developed. High activity of lipolytic enzyme was obtained by first defatting the rice bran to remove lipid components with various extraction conditions. Then, after five cycles of aqueous extraction, rice bran lipolytic enzyme was purified using micro- and ultrafiltration apparatus. Lipolytic enzyme activity was estimated by its hydrolytic action of tributyrin. The result indicated that the standard activity curve of butyric acid showed that the potential rice bran enzyme is a hydrolytic lipase enzyme. In addition, it showed higher lipolytic activity and specific enzyme activity with further purification by micro- and ultrafiltration.
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