고려인삼으로부터 Peroxidase 유전자의 Cloning 및 Activity

  • 손화 (경희대학교 생명과학대학 한방재료가공학과) ;
  • 심주선 (경희대학교 생명과학대학 한방재료가공학과) ;
  • 양덕춘 (경희대학교 생명과학대학 한방재료가공학과)
  • Published : 2004.12.10

Abstract

A peroxidase[E.C.1.11.1.7] is very important enzymes,e.g., as preventive antioxidants. The function is connected with growth and specialization of plant. It makes from the peroxidase and other product to save itself When a plant have been under stress of environment. A class III peroxidase cDNA was isolated from the flower bud of Panax ginseng C.A. Meyer and named PgPrx3. The PgPrx3 is an ORF(open reading frame) of 1,065 bp and a amino acid of 355 residue. Used BioEdit software to compare the PgPrx3 amino acid sequence with other plants which have already known a result of Identity was Spinacia oleracea(70%), vigna angularis(71%), Nicotiana tabacum(69%) and Linum usitatissimym(65%). The peroxidase of Vigna angularis has high homology relationship with ginseng. for that reason, the PgPx3 is a member of class III peroxidase.

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