한국가금학회:학술대회논문집 (Proceedings of the Korea Society of Poultry Science Conference)
- 한국가금학회 2004년도 제21차 정기총회 및 학술발표회
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- Pages.9-10
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- 2004
Development of novel markers for the characterization of chicken primordial germ cells
- Lee, Bo-Ram (Division of Animal Genetic Engineering, School of Agricultural Biotechnology, Seoul National University) ;
- Kim, Duk-Kyung (Division of Animal Genetic Engineering, School of Agricultural Biotechnology, Seoul National University) ;
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Lee, Young-Mok
(Division of Animal Genetic Engineering, School of Agricultural Biotechnology, Seoul National University) ;
- Jung, Jin-Gyoung (Avicore Biotechnology Institute Inc.) ;
- Kim, Jin-Nam (Avicore Biotechnology Institute Inc.) ;
- Lee, Seon-Duk (Avicore Biotechnology Institute Inc.) ;
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Park, Tae-Sub
(Division of Animal Genetic Engineering, School of Agricultural Biotechnology, Center for Agricultural Biomaterials, Seoul National University) ;
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Lim, Jeong-Mook
(Division of Animal Genetic Engineering, School of Agricultural Biotechnology, Seoul National University) ;
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Han, Jae-Yong
(Division of Animal Genetic Engineering, School of Agricultural Biotechnology, Seoul National University)
- 발행 : 2004.11.01
초록
형질전환 가금의 생산은 생체반응기(Bioreactor)가금에 의한 고부가가치의 생의약 물질을 저비용, 고효율로 생산할 수 있으며, 배 발달과정 및 유전자 조절기작 규명을 통한 학문적 이용성 등 다양한 분야에 응용될 수 있다. 형질전환 가금을 생산하기 위한 방법 중 닭의 배 발생 초기에 발생하는 성세포(정자 혹은 난자)의 전구세포인 원시생식세포를 이용한 연구가 활발하게 진행되고 있다. 그러나 이를 검증할 원시생식세포 특이적 마커의 부재로 많은 어려움을 겪고 있다. 따라서 본 연구는 원시생식세포의 특성 분석을 위해 PAS(Periodic acid-Schiff) 염색 및 특이항체 (SSEA-1,3,4 & Integrins
We developed a new panel of markers for the characterization of chicken PGCs. The results of immunostaining demonstrated that anti-SSEA-3, anti-SSEA-4, anti-integrin 6, and anti-integrin 1 antibodies. and STA and DBA bound specifically to chicken PGCs. These reagents could be used to characterize chicken PGCs together with conventional marker reagents such as PAS and anti-SSEA-1 antibody. We also showed that double staining of PGCs with the newly developed markers was feasible, which might contribute to rapid detection and accurate characterization of chicken PGCs.