한국미생물생명공학회:학술대회논문집 (Proceedings of the Korean Society for Applied Microbiology Conference)
- 한국미생물생명공학회 2004년도 Annual Meeting BioExibition International Symposium
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- Pages.297-301
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- 2004
Cell Cycle Arrest and Apoptotic Induction by MCS-C2 in Human Leukemia HL-60 Cells
- Kim, Min-Kyoung (Department of Medical Genetics & Institute of Biomedical Science, College of Medicine, Hanyang University) ;
- Lee, Chul-Hoon (Department of Medical Genetics & Institute of Biomedical Science, College of Medicine, Hanyang University)
- 발행 : 2004.06.01
초록
The purpose of the present study was to investigate the anti-proliferative and apoptotic effects of MCS-C2, a novel analogue of toyocamycin and sangivamycin, in human promyelocytic leukemia (HL-60) cells. When treated with MCS-C2, inhibited proliferation associated with cell cycle arrest and apoptotic induction was found in the HL-60 cells in a concentration-dependent and time-dependent manner. This apoptotic induction was associated with the cleavage of Bid and a release of cytochrome c from mitochondria into the cytosol, followed by the activation of caspase-3 and inactivation of poly (ADP-ribose) polymerase (PARP). However, there was no significant change in any other mitochondrial membrane proteins, such as Bcl-2 and Bax. Consequently, the current findings suggest that the mitochondrial pathway was primarily involved in the MCS-C2-induced apoptosis in the human promyelocytic leukemia HL-60 cells.
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