Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)

The Korean Society of Animal Reproduction (한국동물번식학회)
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Calcium Signaling of M II Oocyte during Chemical Activation of Calcium Ionopore and Cytochalasin B

  • Kim, Sung-Woo (Division of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Park, Jin-Ki (Division of Animal Biotechnology, National Livestock Research Institute, RD) ;
  • Park, Chun-Gyu (Division of Animal Biotechnology, National Livestock Research Institute, RDA, Department of Animal Science, Graduate School, Konkuk University) ;
  • Lee, Ju-Young (Division of Animal Biotechnology, National Livestock Research Institute, RD) ;
  • Han, Joo-hee (Division of Animal Biotechnology, National Livestock Research Institute, RDA, Department of Animal Science, Graduate School, Konkuk University) ;
  • Lee, Seung-Eun (Division of Animal Biotechnology, National Livestock Research Institute, RDA, Department of Animal Science, Chungbuk National University) ;
  • Baek, Kyung-Nye (Division of Animal Biotechnology, National Livestock Research Institute, RD) ;
  • Chang, Won-Kyung (Division of Animal Biotechnology, National Livestock Research Institute, RDA)
  • Published : 2004.06.01
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Abstract

The calcium ionophore (A23187) has been used for activation of porcine oocytes from in vitro maturation by many researches. The signaling of calcium was known to be a primary factor of activation of MII oocyte by calcium ionophore. The calcium level was measured by an intensity of fluo 4 fluorescence and confocal microscope. The level was increased by 7% ethanol or 70 μM calcium ionophore but oscillation was not found. (omitted)

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