Parthenogenetic Activity of Porcine Sperm Factor to In Vitro Matured Porcine Oocyte

  • Park, Chun-Gyu (Division of Animal Biotechnology, National Livestock Research Institute, RDA, Department of Animal Science, Graduate School, Konkuk University) ;
  • Park, Jin-Ki (Division of Animal Biotechnology, National Livestock Research Institute, RD) ;
  • Kim, Sung-Woo (Division of Animal Biotechnology, National Livestock Research Institute, RD) ;
  • Lee, Ju-Young (Division of Animal Biotechnology, National Livestock Research Institute, RD) ;
  • Han, Joo-hee (Division of Animal Biotechnology, National Livestock Research Institute, RDA, Department of Animal Science, Graduate School, Konkuk University) ;
  • Lee, Seung-Eun (Division of Animal Biotechnology, National Livestock Research Institute, RDA, Department of Animal Science, Chungbuk National University) ;
  • Baek, Kyung-Nye (Division of Animal Biotechnology, National Livestock Research Institute, RD) ;
  • Chang, Won-Kyung (Division of Animal Biotechnology, National Livestock Research Institute, RDA)
  • 발행 : 2004.06.01

초록

Porcine sperm extract (PSE) supporting Ca/sup 2+/ osillation was microinjected into the in vitro matured porcine oocytes. In the presence of the capacitative Ca/sup 2+/ entry mechanism which can activate MII oocytes, preparation methods of sperm extraction were studied by many researchers. Such as freeze-thaw cycle, homogenation, sonication of boar sperm was used for certification of their activity of calcium signals. (omitted)

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