Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2004.06a
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- Pages.231-231
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- 2004
Identification of Differential Expressed Genes at 2-cell Stage Porcine Embryo using ACP-based DD-RT-PCR
- Hwang, Kyu-Chan (Department of Animal Science, Chungbuk National University, Division of Applied Life Science, Gyeongsang National University) ;
- Cui, Xiang-Shun (Department of Animal Science, Chungbuk National University) ;
- Lee, Hwa-Young (Department of Animal Science, Chungbuk National University) ;
- Jin, Yong-Xun (Department of Animal Science, Chungbuk National University) ;
- Kim, Jin-Hoi (Division of Applied Life Science, Gyeongsang National University) ;
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Kim, Nam-Hyung
(Department of Animal Science, Chungbuk National University)
- Published : 2004.06.01
Abstract
Successful embryonic development is dependant on temporal and stage-specific expression of appropriate genes. However, information on specific gene expression during early cleavage before zygotic gene activation (ZGA) is lacking. In the present study, we compared gene expression between porcine parthenotes 2-cell and blastocyst embryos to identify the genes that are specifically or prominently expressed by employing annealing control primers (ACP)-based Gene Fishing RCR. (omitted)