Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2004.06a
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- Pages.198-198
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- 2004
Differentially Expressed Genes in Porcine Parthenogenetic 2-cell and Blastocyst Embryos
- Lee, Hwa-young (Department of Animal Science, Chungbuk National University, Animal Biotechnology Division, National Livestock Research Institute, Rural Development Adminstration) ;
- Kim, Bong-Ki (Animal Biotechnology Division, National Livestock Research Institute, Rural Development Adminstratio) ;
- Cui, Xiang-Chun (Department of Animal Science, Chungbuk National University) ;
- Hwang, Kyu-Chan (Department of Animal Science, Chungbuk National University) ;
- Chung, Hak-Jae (Animal Biotechnology Division, National Livestock Research Institute, Rural Development Adminstratio) ;
- Ko, Yeoung-Gyu (Animal Biotechnology Division, National Livestock Research Institute, Rural Development Adminstratio) ;
- Jung, Jin-Kwan (Animal Biotechnology Division, National Livestock Research Institute, Rural Development Adminstratio) ;
- Chang, Won-Kyong (Animal Biotechnology Division, National Livestock Research Institute, Rural Development Adminstratio) ;
- Kim, N. H. (Department of Animal Science, Chungbuk National University)
- Published : 2004.06.01
Abstract
The identification of embryo-specific genes would provide insights into early embryonic development. However, the current methods employed to identify the genes that are expressed at a specific developmental stage are labor intensive and suffer from high rates of false positives. Here we employed a new and accurate reverse transcription-polymerase chain reaction (RT-PCR) technology that involves annealing control primers(ACPs) to identify the genes that are specifically expressed in porcine blastocysts compared to 2-cell stage embryos. (omitted)