한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Process to industrial production of xylanase from recombinant Bacillus subtilis DB431

  • Choi, Young-Rok (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Seo, Eun-Jin (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Nam, Soo-Wan (Department of Biotechnology and Bioengineering, Dong-Eui University) ;
  • Kwon, Hyun-Ju (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Kim, Byung-Woo (Department of Life Science and Biotechnology, Dong-Eui University)
  • Published : 2003.10.22
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Abstract

A strong constitutive $P_{JH}$ promoter from Bacillus was applied to overexpress the end oxylanase gene in Bacillus subtilis. The expression plasmid, pJHKJ4, was designed to contain the $P_{JH}$ promoter and endoxylanase promoter $(P_B)$, and introduced into Bacillus subtilis DB431 The total activities of the enzymes reached about 140 unit/ml by cultivation of B. subtilis DB431 harboring pJHKJ4 in LB glucose medium. Ultrafilteration is effective its yield is 70%.

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