한국생물공학회:학술대회논문집
- 2003.10a
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- Pages.468-472
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- 2003
Yeast cell surface display of cellobiohydrolase I
- Lee, Sun-Kyoung (Bioprocessing Research Laboratory, Dept. of Chemical Engineering, Hanyang University) ;
- Suh, Chang-Woo (Bioprocessing Research Laboratory, Dept. of Chemical Engineering, Hanyang University) ;
- Hwang, Sun-Duk (Nano-Bio Technology Lab., Dept. of Chemical Engineering, Hannam University) ;
- Kang, Whan-Koo (Nano-Bio Technology Lab., Dept. of Chemical Engineering, Hannam University) ;
- Lee, Eun-Kyu (Bioprocessing Research Laboratory, Dept. of Chemical Engineering, Hanyang University)
- Published : 2003.10.22
Abstract
Recently, genetic engineering techniques have been used to display various heterologous peptides and proteins (enzyme, antibody, antigen, receptor and fluorescence protein, etc.) on the yeast cell surface. Living cells displaying various enzymes on their surface could be used repeatedly as 'whole cell biocatalysts' like immobilized enzymes. We constructed a yeast based whole cell biocatalyst displaying T. reesei cellobiohydrolase I (CBH I ) on the cell surface and endowed the yeast-cells with the ability to degrade cellulose. By using a cell surface engineering system based on
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