Isolation of antifungal compounds from Helicosporium nizamabadense KCTC 0635BP

  • Kim, Ki-Wook (Institute of Genetic Engineering, Changwon National University) ;
  • Bae, Yun-Ui (Institute of Genetic Engineering, Changwon National University) ;
  • Yoo, Ju-Soon (Institute of Genetic Engineering, Changwon National University) ;
  • Cho, Su-Dong (Institute for Basic Science, Changwon National University) ;
  • Moon, Ja-Young (Institute of Genetic Engineering, Department of Biochemistry and Health Sciences, Changwon National University) ;
  • Kim, Dong-Wan (Department of Microbiology, Changwon National University) ;
  • Jeong, Yong-Kee (Department of Microbiology, Dong-Eui University) ;
  • Joo, Woo-Hong (Institute of Genetic Engineering, Department of Biology, Changwon National University)
  • Published : 2003.10.22

Abstract

One strain was isolated from wilted chestnut tree and identified as Helicosporium nizamabadense. Also, the strain was evaluated for biocontrol potentials against phytopathogens. Autoclaved fraction of the isolate also showed complete growth inhibition of the turfgrass large patch pathogen, Rhizoctonia. solani AG2-2 and plant pathogen, Botrytis cinerea. Culture extracts of Helicosporium nizamabadense were purified by Thin Layer Chromatography. The several fractions were analyzed by GC-MS, and NMR etc. Antifungal activities were presumably showed more than 5 fractions against the pathogens. Among them, the most effective antifungal fraction was identified as 2-Methylresocinol using GC-MS, and NMR. These results suggest that the 2-Methylresocinol may be useful for biochemicals.

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