한국생물공학회:학술대회논문집
- 한국생물공학회 2003년도 생물공학의 동향(XIII)
- /
- Pages.341-345
- /
- 2003
Immobilization of transgenic Nicotiana tabacum cell suspensions for the continuous production of hGM-CSF
- Roh, Yun-Sook (Department of Biological Engineering, Inha University) ;
- Lee, Sang-Yoon (Department of Biological Engineering, Inha University) ;
- Kim, Dong-Il (Department of Biological Engineering, Inha University)
- 발행 : 2003.10.22
초록
형질전환된 담배세포 배양을 이용한 hGM-CSF 생산에 있어 polyurethane foam 내 고정화법을 이용하여 연속배양의 가능성을 확인하였고 spinner flask에서의 배양에 따른 영향을 알아보았다. 16일 동안 3번의 배지교환에도 세포의 활성이 유지되어 hGM-CSF 생산량은 계속적으로 증가하였다. 온도와 교반속도를 동일하게 하였을 때 spinner flask에서의 hGM-CSF 생산량은 17.3
Effect of immobilization on the production of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) by Nicotiana tabacum cells was investigated using polyurethane foam as immobilization matrices. The cell activity and the hGM-CSF production were maintained for 16 days in spite of 3 times of media exchange. Under the same conditions of temperature and agitation rate, maximum concentrations of hGM-CSF in a 500-mL spinner flask and 100-mL Erleuneyer flasks were 17.3
키워드