Proceedings of the Korean Society of Life Science Conference (한국생명과학회:학술대회논문집)

Korean Society of Life Science (한국생명과학회)
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Preparation and Characterization of ${\alpha}$-D-Glucopyranosyl- ${\alpha}$-Acarviosinyl-D-Glucopyranose, a Novel Inhibitor Specific for Maltose-Producing Amylase

  • Kim, Myo-Jeong (Food Science Institute and School of Food and Life Science, Inje University) ;
  • Park, Kwan-Hwa (Research Center for New Bio-Materials in Agriculture and Department of Food Science & Technology, School of Agricultural Biotechnology, Seoul National University)
  • Published : 2003.05.23
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Abstract

A novel inhibitor against maltose-producing a-amylase was prepared via stepwise degradation of a high molecular weight acarbose (HMWA) using Thermus maltogenic amylase (ThMA). The structure of the purified inhibitor was determined to be ${\alpha}$-D-glucopyranosyl-${\alpha}$-acarviosinyl-D-glucopyranose (GlcAcvGlc). Progress curves of p-nitrophenyl-${\alpha}$-D-maltoside (PNPG2) hydrolysis by various amylolytic enzymes, including maltogenase (MGase), ThMA, and cyclodextrinase(CDase) I-5, in the presence of acarbose or GlcAcvGlc indicated a slow-binding mode of inhibition. The inhibition potency of GlcAcvGlc for MGase, ThMA, and CDase I-5 was 3 orders of magnitude higher than that of acarbose.

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