한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Molecular cloning and characterization of ornithine decarboxylase gene from flounder (Paralichthys olivaceus)

  • Son, Mi-Young (Department of microbiology, Pukyong National University) ;
  • Lee, Jae-Hyung (Department of microbiology, Pukyong National University) ;
  • Lee, Moo-Hyung (Department of microbiology, Pukyong National University) ;
  • Kim, Young-Tae (Department of microbiology, Pukyong National University)
  • Published : 2003.04.11
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Abstract

Ornithine decarboxylase (ODC) is the key enzyme in the synthetic pathway of polyamines. This enzyme is a homodimeric and a pyridoxal 5-phosphate (PLP) dependent enzyme. We have isolated, a cDNA clone encoding ODC from brain cDNA library constructed from flounder (Paralichthys olivaceus). The ODC cDNA contained a complete ORF consisting of 460 amino acids and one stop codon with comparison to nucleotide sequences of the flounder, zebrafish and rat ODC genes, the ODC genes were highly conserved. The transcription of ODC was analyzed with reverse transcription-polymerase chain reaction (RT-PCR) species in brain, kidney, liver, and embryo.

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