Studies on the Effective Drug Delivery System Using Naked Plasmid DNA for the Erythropoietin Expression in vivo

  • 박영섭 (서울대학교 협동과정생물화학공학) ;
  • 정동건 (서울대학교 응용화학부) ;
  • 안진호 (서울대학교 협동과정생물화학공학) ;
  • 최차용 (서울대학교 협동과정생물화학공학, 서울대학교 응용화학부) ;
  • 주현 (아주대학교 생명과학과)
  • Published : 2003.04.11

Abstract

There has been interest in developing gene therapy based on naked plasmid DNA for treating serum protein deficiencies and human erythropoietin (hEPO) is one of the candidate for gene therapy being Investigated most enthusiastically. We constructed novel plasmid DNA vectors pVAC-hEPOI/II/III which contain one, two, three hEPO gene(s) respectively for producing high level expression and secretion of hEPO in vitro and in vivo. NIH3T3 and COS7 cells were transfected transiently with these vectors and increase in hEPO expression in medium reached 2-5 fold in comparison with pSecTagB-hEPO. Intra muscular administrations of pVAC-hEPOI/II/III vectors into mice resulted in high level secretion of hEPO in the serum and corresponding increases in hematocrit level. In conclusion the transduction efficiency of naked plasmid vectors is one of the critical factors of a gene delivery system and these novel plasmid vectors will contribute to various gene therapy based on naked plasmid DNA.

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