한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Development of Doxorubin Overproducing Streptomyces Using Protoplast Regeneration

  • 박희섭 (인하대학교 공과대학 생물공학과 미생물분자생물공학 실험실) ;
  • 박현주 (인하대학교 공과대학 생물공학과 미생물분자생물공학 실험실) ;
  • 김응수 (인하대학교 공과대학 생물공학과 미생물분자생물공학 실험실)
  • Published : 2003.04.11
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Abstract

To establish an effective and reliable technique of mutation by protoplast regeneration in doxorubicin overproducing industrial strain, it is essential to optimize the conditions for protoplast regeneration. $CaCO_3$ as buffer, the negative effect of glucose was still evident without significant changes in pH, ruling out acidity as responsible for the suppression of anthracycline production and suggesting a direct effect of glucose on antibiotic biosynthesis. Production of doxorubicin was improved in doxorubicin overproducing industrial strain (BR-Dox) when protoplast regenerated. BR-Dox4 and BR-Dox6 of BR-Dox derivatives improved doxorubicin production by 25.2 % and 12.2 %, respectively.

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